Mutations from IPEX patients elicit a range of phenotypes in mice [RNAseq]

RNAseq analysis of mouse Treg from Foxp3 missense mutant mice hemizygous male and heterozygous female : R51Q, C168Y, K199del, R309Q, F324L, R337Q Overall design: Mouse CD4+ regulatory Tregs in 6-10 weeks old Foxp3 missense mutant mice hemizygous male and heterozygous female were isolated from spleen by flow cytometry, as DAPI–CD19-TCRß+CD4+CD25+. 1,000 cells were double-sorted directly into lysis buffer for RNA-seq (Smart-seq2) library preparation following the standard ImmGen low-input protocol (www.immgen.org).