Single-cell RNA-sequencing analysis of the IL-10 dependent response of human CD14+ monocytes to LPS

Inflammatory bowel diseases (IBD) involve dysregulated immune responses. Understanding the cellular specificity and compartmentalisation of cytokine responses may help to target cytokine therapies. Intestinal inflammation involves influx of CD14+ monocytes into the lamina propria of the gut where they can adopt pro-inflammatory phenotypes. In the healthy intestine, IL-10 plays a key role in controlling inflammation and promoting homeostasis. This data was generated to investigate the phenotypes that CD14+ monocytes can adopt following LPS stimulation with or without concominant IL-10 signaling blockade. Overall design: CD14+ monocytes were isolated from a healthy human donor and subject to droplet based single-cell RNA-sequencing after exposure to conditions of (i) no stimulation, (ii) stimulation with LPS or (iii) stimulation with LPS + anti-IL-10R antibody. The stimulation with LPS + anti-IL10R antibody was repeated with cells from a second healthy donor.