The transcriptomic landscape of glutamatergic cortical neurons carrying Alzheimer's disease PSEN1 mutations reveals alterations in structural components, signalling, and ncRNA regulation

To investigate gene expression changes related to two fAD mutations (A79V and L150P) in the Presenilin-1 gene (PSEN1) we compared the transcriptomes (polyA and total) of glutamatergic cortical neurons derived from fAD-mutant human induced pluripotent stem cells and their individual isogenic controls generated via precision CRISPR/Cas9 genome editing. Overall design: RNA-seq study on human induced pluripotent stem cells differentiated in glutamatergic cortical neurons carrying A79V and L150P mutations in PSEN1, compared to gene-corrected controls.