Neurospora crassa MNase (Micrococcal nuclease) seq. Neurospora crassa strain:87-3

Neurospora was cultured in petri dishes in liquid medium for two days.Afterwards, the Neurospora mats were cut into discs and transferred into medium-containingflasks and were harvested at DD14. After crosslinking with 1% formaldehyde for 30 min atroom temperature, nuclei were incubated with micrococcal nuclease (Takara) for 60 min at 37 °Cbefore the reaction was stopped by addition of EDTA. Sequencing libraries were generated using50 ng of DNA purified from the MNase-digested chromatin (Diagenode DNA sample prep kit)and size-selected by agarose gel purification to ensure insert sizes close to that of DNA bound bymononucleosomes. After 75bp paired-end sequencing, the MNase-seq data were normalized bytotal number of reads.