RNA sequencing analysis of HHV-6A-infected T cells

To determine if HHV-6A infection affects cell metabolism of host cells, we conducted a global RNA sequencing analysis in HHV-6A infected cells Overall design: Mock-infected and HHV-6A-infected HSB-2 cells were cultured for 72 h. Total RNA was extracted using TRIzol reagent (Invitrogen, USA) following the manufacturer's instructions. A total amount of 3 µg of RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer's recommendations. After cluster generation, the library preparations were sequenced on an Illumina Hiseq 2500 platform by CapitalBio Technology. Differentially expressed genes were identified by fold-change screening. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed to systematically identify differentially expressed genes and the pathways associated with HHV-6A infection.