Fig_8_behavior_temperature_bodyweight

Mice were subjected to 30 min MCAo or sham surgery and TAT.b-Gal or TAT.ARC were administered in the contralateral ventricle after 3h after the onset of ischemia and mice were observed over 28 days. DeSimoni Neuroscore was performed at indicated time points (Fig. 8) as described (De Simoni et al., 2003; Orsini et al., 2012) with some modifications. In brief, general health (Table 2) and specific focal assessments (Table 3) were separately scored, analyzed and finally added to form a summation score. Summative scores added up to a maximum of 43 points with more points meaning more deficits. Rota rod was assessed as described recently (Hoffmann et al., 2015) and the best run out of three replicates at a given time point was used for statistical analysis. Body temperature was measured daily as survival was documented. Body temperature was non-invasively assessed at the same time of the day prior to body weight measurements using subcutaneous transponders (IPTT-300, Bio Medic Data Systems), for unambiguous identification of mice in their home cages as described (Kort et al., 1998; Langer and Fietz, 2014).