Gene expression profile of kidney and lung cells at 24 hours after the onset of renal bilateral ischemia-reperfusion injury

Remote lung injury after acute kidney inkury (AKI) is a common issue in humans and is lethal especially in critically-ill patients. We investigated kidney-lung interactions following AKI using single-cell RNA sequencing (scRNA-seq) of kidney and lung in a murine AKI model. Overall design: Single cell suspensions from lungs and kidneys were generated from mice with AKI (bilateral ischemia reperfusion injury) or control (sham surgery). Samples from 4 animals were pooled in each condition, subsequently processed, sequenced, and analyzed.