Comparison of the new tSEM versus TEM systems.

aAt the time of this writing, Zeiss is the sole source of the FE-SEM system designed for high-resolution large-field transmission imaging of biological samples as described here.bTEM can be retrofitted to acquire mosaic images automatically with open source software such as Leginon (National Resource for Automated Molecular Microscopy, The Scripps Research Institute; http://www.leginon.org/) and SerialEM (The Boulder Lab for 3D Electron Microscopy, University of Colorado; http://bio3d.colorado.edu/SerialEM/).cNote that the operator time for tSEM remains constant for the same number of serial sections, even when the volume size is increased by montaging.