M6A IP from BrU-labeled nascent RNA (non-fragmented).

M6A immunoprecipitation from 15 min BrU-labeled nascent RNA (0 min and 30 min chase). All three fractions, Input, eluate and supernatant were subjected to Illumina sequencing. HEK293T cells were incubated with 2 mM bromouridine for 15 min labeling time (pulse) and chased with 20 mM uridine for 0 min or 30 min. Whole-cell total RNA was extracted with Trizol and nascent RNA was purified with anti-BrdU during BrU-IP. The BrU-labeled nascent RNA was subsequently immunoprecipitated with anti-m6A. The Input, m6A-IP eluate (m6A-enriched RNA fraction) and supernatant (m6A-depleted RNA fraction) were subjected to Illumina sequencing.