Data set associated to simulations performed within the manuscript "Endosperm turgor pressure both promotes and restricts seed growth and size".

This dataset contains the following files

2F4-Col0-iku2-Fig.3f and Supp. Fig.9

Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose unesterified pectins are labelled with the 2F4 antibody (channel 2)

2 genotypes: Col0 and iku2

3 independent experiments

JIM5-Col0-ap2-Fig. 4d and Supp Fig. 12b

Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose demethylesterified pectins are labelled with the JIM5 antibody (channel 2)

2 genotypes: Col0 and ap2-6

2 independent experiments

JIM5-Col0-iku2-Fig. 3h and Supp Fig. 10

Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose demethylesterified pectins are labelled with the JIM5 antibody (channel 2)

2 genotypes: Col0 and iku2

3 independent experiments

LM19-Col0-ap2-Fig. 4b and Supp Fig. 12a

Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose demethylesterified pectins are labelled with the LM19 antibody (channel 2)

2 genotypes: Col0 and ap2-6

2 independent experiments

LM19-Col0-iku2-Fig. 3d and Supp Fig. 8

Slices of seeds from 3 to 9 Days post-anthesis (DPA) stained with calcofluor (channel 1) and whose demethylesterified pectins are labelled with the LM19 antibody (channel 2)

2 genotypes: Col0 and iku2

3 independent experiments

pELA1-VENUS-Col0-iku2-Fig3c

Confocal stack of developing seeds expressing pELA1::3X-VENUS-N7

2 genotypes: Col0 and iku2

1 independent experiments

pELA1-VENUS-Col0-iku2-Supp Fig. 6

Confocal stack of developing seeds expressing pELA1::3X-VENUS-N7

2 genotypes: Col0 and iku2

1 independent experiments

Seed-size-ap26-iku2-Fig.5e-Supp-Fig. 12c

Pictures of dry seeds

4 genotypes: Col0, iku2, ap2-6 and iku2 ap2-6

2 independent experiments

Wall-rupture-Col0-iku2-Fig3k

Confocal stack of developing seeds expressing LTi6b-GFP (First channel) and dyed with FM4-64 (second channel) imaged after a 40 µm indentation to break testa wall

2 genotypes: Col0 and iku2

2 replicates

Wall-rupture-Col0-iku2-Supp Fig. 11

Confocal stack of developing seeds expressing LTi6b-GFP (First channel) and dyed with FM4-64 (second channel) imaged after a 30 µm or a 50µm indentation to break testa wall

2 genotypes: Col0 and iku2

2 replicates

181211-Col0-iku2-Timelapse-Seed-Size

Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10 DPA

2 genotypes: Col0 and iku2

190125-Col0-iku2-Timelapse-Seed-Size

Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10 DPA (no 9DPA)

2 genotypes: Col0 and iku2

190523-Col0-ede13-Timelapse-Seed-Size

Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 9DPA

2 genotypes: Col0 and ede1-3

200724-Col0-iku2-Timelapse-Seed-Size

Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10DPA

2 genotypes: Col0 and iku2

200921-Col0-iku2-Timelapse-Seed-Size

Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10DPA

2 genotypes: Col0 and iku2

201023-Col0-ede13-Timelapse-Seed-Size

Pictures of developing seeds from 0 (ovules) days post-anthesis (DPA) to 10DPA

2 genotypes: Col0 and ede1-3

210519-Col0-Timelapse-invitro-Sorbitol

Picture of developing WT seeds (Col-0) at 3, 6, 9 and 12DPA

Fruits were grown in planta (uncut) or in vitro from 3DPA onwards

Fruits growing in vitro were cultivated in ½ MS + 1% Sucrose + 0.1X PPM (Plant Preservative Medium) + 1X Gamborg Vitamins + 0 to 200mM Sorbitol

Note that the file containing the pictures from this experiment was too large for Zenodo, the pictures were thus transformed into 8 bit black/white pictures, a 2x2 binning was applied, before a saving into tiff. The original pictures are available at: http://flower.ens-lyon.fr/

210709-Col0-Timelapse-invitro-Sorbitol

Picture of developing WT seeds (Col-0) at 3, 6, 9 and 12DPA

Fruits were grown in planta (uncut) or in vitro from 3DPA onwards

Fruits growing in vitro were cultivated in ½ MS + 1% Sucrose + 0.1X PPM (Plant Preservative Medium) + 1X Gamborg Vitamins + 0 to 200mM Sorbitol

Note that the file containing the pictures from this experiment was too large for Zenodo, the pictures were thus transformed into 8 bit black/white pictures, a 2x2 binning was applied, before a saving into tiff. The original pictures are available at: http://flower.ens-lyon.fr/

data.zip file:

Measurements of seed growth tracking experiments performed on WT plants as well as iku2 and ede1-3 mutants.

Measurements of endosperm pressure performed on WT plants and iku2 mutants.

Simulation results from a parameter space exploration of the system of ODEs we studied within the scope of the work described in the manuscript.