Functional Analysis of L Protein Mutants in LCMV Mini-Replicon System.

1Mutants with selective defect in mRNA synthesis are shown in boldface. 2Standardized relative light unit (sRLU) value (wild-type = 100%). Mean of ≥2 independent transfection experiments. 3sRLU value of mutant divided by sRLU value of negative control mutant containing a mutation in the catalytic site of the RNA-dependent RNA polymerase. Mean of ≥2 independent transfection experiments. 4Antigenome signals in Northern blots were quantified via intensity profiles (wild-type = 100%). 5RNA signals in Northern blots were quantified and the mRNA-to-antigenome signal ratio was calculated. The wild-type ratio was set at 1 for each experiment (i.e. the signal ratio of a mutant was normalized with the wild-type ratio) to render independent blots comparable.