Three independent benzimidazole resistance mutations in a single Ancylostoma caninum population from Sao Paulo, Brazil

This BioProject contains raw amplicon sequencing reads generated from fecal total nucleic acids from a canine breeding kennel in Sao Paulo, Brazil. The study investigates benzimidazole resistance in the canine hookworm Ancylostoma caninum by sequencing the isotype-1 beta-tubulin gene regions spanning resistance-associated codons 134/167 and 198/200 using Illumina MiSeq, and by sequencing a near full-length beta-tubulin amplicon using Oxford Nanopore Technologies to assess haplotypes.ITS2 amplicon sequencing data are included to confirm species identity. The dataset supports analysis of resistance-associated SNPs (including Q134H, F167Y, and F200Y).