Lipid set enrichment analysis using plasma lipidomics data from the Australian Diabetes Obesity and Lifestyle Study.

Lipidomic analysis was performed on the AusDiab cohort (n = 10,399). Enrichment scores were generated by summing the association t-statistics for individual lipids against waist circumference (WC). 1Lipid sets denote pre-defined lipid groups used in the analysis. Lipid domains map to lipid species containing the same backbone, i.e., Sphingolipids; lipids containing a spingoid base; Glycerophospholipids; lipids containing a glycerol base with a phospho-head group; Neutral lipids: sterol lipids and lipids containing a glycerol backbone; lipid subclasses are clustered by unique structural features; lipid classes include all lipids with the respective headgroup; lipid features include any lipid species containing the respective fatty acid at the sn-1, sn-2 or sn-3 position; 2LSEA Score: represents the correlation adjusted sum of the t-statistics for individual lipids against WC. 3BH: corrected p-values using the false discovery rate method of Benjamini and Hochberg. (XLSX)