Dataset for: Bacteriocin ASM1 is an O/S-diglycosylated, plasmid-encoded homologue of glycocin F

<i>Lactobacillus plantarum</i> A-1 secretes bacteriocin ASM1, with a calculated monoisotopic mass of 4642.8454 Da (based on the 43 amino acid sequence) and a measured mass of 5044.9727 Da. Here we show that ASM1 is a glycocin (glycosylated bacteriocin) with <i>O</i>- and <i>S</i>-linked <i>N</i>-acetylglucosamine moieties identical to glycocin F (GccF), and that the roles of specific structural features of ASM1 are very similar to GccF. Analysis of <i>Lb. plantarum</i> A-1 genomic DNA sequences showed that <i>asm</i> gene organisation (<i>asmH←→asmABCDE→F</i>) is identical to the <i>gcc</i> gene cluster, and that the <i>asm</i> genes reside on the novobiocin-curable 11,905-bp plasmid pA1_ASM1. Amino acid sequence identity averages ~69% across all Asm and Gcc proteins, and is highest for the identical ASM1/GccF peptide scaffold residues 1-30.