transcriptional sequencing of Nipponbare under salt stress

Nipponbare rice seed was immersed in distilled water in dark room, and the uniformly germinated seeds were sown in 96-well plates supported by a plastic container. The seeds were then grown in the artificial climate chamber, the growth medium was updated every 3 days. After 14 days, the salinity stress was conducted by treating the Nipponbare seedlings with 200 mM NaCl medium for 3 days. Subsequently, the shoot and root samples were collected and immediately frozen in liquid nitrogen. For RNA extraction, 10 plants were collected and mixed to minimize the effect of transcriptome unevenness among different plants. Three biological repeats were taken from each group of samples and transcriptome sequencing was performed on an Illumina Hi-seq 2000 Sequencer.