Differential Gene Expression in Tumor vs Adjacent Normal in Stomach Adenocarcinoma Patients

After radical resection, tumor (Stomach Adenocarcinoma) and adjacent normal tissues were collected in RNALater and stored at -80 °C temperature for RNA isolation. After RNA Isolation, RNA Integrity Number ≥ 6 was selected for cDNA library construction. The clusters were generated on a cBot cluster generation system (Illumina) and paired-end 2 x 100 bp sequencing was performed in Illumina HiSeq 2500 platform. The fastq files were aligned to the GRCh38 reference genome using STAR aligner to generate the BAM files. The generated data were used for differential gene expression study.