Bulk RNA-seq on NOD mice prostate tissues treated with etanercept. Bulk RNA-seq on NOD mice prostate tissues treated with etanercept

Immunohistochemistry studies indicated that etanercept treatment reduced inflammation, epithlial proliferation, and epithelial NFkB activity in NOD prostate tissues compared to control treated mice. A bulk RNA-seq study was conducted on whole mouse prostate tissues from NOD mice treated with PBS control or etanercept for 5 weeks to identify putative pathways involved in these processes. These analyses determined differentially expressed genes and numerous enriched pathways yielding a greater understanding of pathways involved in prostate regression and inhibition of inflammation. Overall design: whole prostate tissues from 4 control or 4 etanercept-treated mice used for RNA isolation, followed by bulk RNA-seq