Miguel Vicente-Manzanares, Karen Newell-Litwa, Alexia I. Bachir, Leanna A. Whitmore, Alan Rick Horwitz (2011) CIL:25847, Cricetulus griseus, epithelial cell. CIL. Dataset

6 MIIB inhibits adhesion turnover. Time-lapse of a CHO.K1 cell cotransfected with very low levels of GFP-MIIB (myosin IIB, green) and paxillin (magenta). Note that adhesions adjacent to MIIB-containing bundles do not disassemble (representative adhesions are marked with yellow arrowheads). Total internal reflection fluorescence (TIRF) images were acquired on an inverted microscope (IX70; Olympus) with a 1.45 NA (oil) Plan-Apochromat 60× TIRFM objective lens. Images were acquired with a charge-coupled device (CCD) camera (Retiga Exi; Qimaging). This movie corresponds to Fig. 5 D and video 6 in J Cell Biol 2011 193:381-396.

6 MIIB抑制了粘附周转。CHO.K1细胞共转染低水平GFP-MIIB（肌球蛋白IIB，绿色）和paxillin（洋红色）的共聚焦时序图像。请注意，位于MIIB富含束附近的粘附并未解离（代表性的粘附以黄色箭头标记）。采用总内反射荧光（TIRF）图像在倒置显微镜（IX70；Olympus）上，配备1.45 NA（油）Plan-Apochromat 60× TIRFM物镜进行采集。图像通过电荷耦合器件（CCD）相机（Retiga Exi；Qimaging）获取。该视频对应于《J Cell Biol 2011 193:381-396》中图5 D和视频6。