Effect of enzymatically-hydrolysed eggshell membrane (eESM) in Human keratinocyte

Skin aging is one of the hallmarks of the aging process that causes physiological and morpho-logical changes. Recently, several nutritional studies were conducted to delay or suppress the aging process. This study investigated whether nutritional supplementation of the eggshell membrane (ESM) has a beneficial effect on maintaining skin health and improving the skin ag-ing process using neonatal normal human epidermal keratinocytes (NHEK-Neo). 1 mg/mL of enzymatically hydrolyzed ESM (eESM) upregulated the expression of keratinocyte differentiation markers, including keratin 1, filaggrin and involucrin, and changed the keratinocyte morphology. Neonatal Normal Human Epidermal Keratinocytes (NHEK-Neo) were incubated with control medium or 1 mg/ml of eESM-supplemented medium for 4 days. N value of each group was three.