single-cell-RNA-sequencing analysis of naive and Listeria monocytogenes-specific CD8+ T cells

The histone methyltransferase Suv39h1 silences transcriptional programs during CD8+-T cell differentiation The study explores the role of gene silencing by Suv39h1, a histone methyltransferase, that plays a critical role in heterochromatin dynamics. Overall design: Naïve littermate and Suv39h1-KO CD8+ T cells, and day 7 p.i. Kb-OVA+ CD8+ T cells (from Listeria m.-OVA infected mice), were isolated by FACS. scRNA-seq was performed according to 10X Genomics technology. Both biological (Rep) and technical (tech) replicates were processed.