Copy number variations may contribute to congenital heart defect risk greatly by disrupting long noncoding RNAs

We identified lncRNAs locating in CNV locus which were coexpressed with multiple congenital heart defect associated genes. To validate our findings, we performed overexpression and knockdown experiments to characterize the trascriptomic profiles regulated by HSALNG0104472. Paired RNA-seq of lncRNA HSALNG0104472 knockdown cardiomyocytes (AC16), HSALNG0104472 overexpression cardiomyocytes, control cardiomyocytes for overexpression of HSALNG0104472 and control cardiomyocytes for knockdown of HSALNG0104472. Three biological replicates were obtained for each sample.