Effects of eCO2 on plant growth and pollen chemistry in 14 angiosperms

Elevated atmospheric carbon dioxide (eCO2) can affect plant growth and physiology, which can, in turn, impact herbivorous insects, including by altering pollen or plant tissue nutrition. Previous research suggests that eCO2can reduce pollen nutrition in some species, but it is unknown whether this effect is consistent across flowering plant species. We experimentally quantified the effects of eCO2 across multiple flowering plant species on plant growth in 9 species and pollen chemistry (%N an estimate for protein content and nutrition in 12 species; secondary chemistry in 5 species) in greenhouses. For pollen nutrition, only buckwheat significantly responded to eCO2, with %N increasing in eCO2; CO2 treatment did not affect pollen amino acid composition but altered secondary metabolites in buckwheat and sunflower. Plant growth under eCO2 exhibited two trends across species: plant height was taller in 44% of species and flower number was affected for 63% of species (3 species with fewer a..., , , # eCO2\_SciReports

Here, we present all code and data files used for analyses and figure making in Bernauer et al. 2024 - *\"[Elevated atmospheric CO2 has small, species-specific effects on pollen chemistry and plant growth across flowering plant species](https://doi.org/10.1038/s41598-024-63967-z)\"*. Access this data set on Dryad: [https://doi.org/10.5061/dryad.70rxwdc5n](https://doi.org/10.5061/dryad.70rxwdc5n).

In total, there are 10 data sets included, which are described in the \"Datasets\" subheading below, as well as seven code files, the first four listed under the \"Analysis and Visualization\" subheading include all manuscript analysis and figure making. In the Greenhouse CO2 Control, we include the scripts used to run the Arduino CO2 controllers within the greenhouse. 

In this study, we examined how CO2 levels influenced plant growth and pollen chemistry (nutrition or %N, secondary metabolites, and amino acids) across fourteen species with known value to pollinators. To do so,...