MicroRNA-138 Suppresses Glioblastoma Proliferation through Downregulation of CD44 (NanoString)

100 ng of total RNA was processed with the Human v3 miRNA Expression Assay using NanoString nCounter system (NanoString, USA). Expression of individual miRNA was measured by quantitative real-time PCR with TaqMan miRNA Expression kit (Applied Biosystems, Thermo Fisher Scientific, USA). This study aimed to identify tumor suppressive miRNAs with a promising therapeutic potential for the treatment of GBM. Our data obtained from miRNA expression profiling on GBM patient samples and patient-derived primary GBM cells have identified miR-138 as one of the most significantly downregulated miRNAs in GBM.