Identifying new signaling mediators responsible for Mesothelin (MSLN) intraperitoneal (IP) tumor growth phenotype

Identification of new targets involved in MSLN signaling by comparing mRNA expression patterns in MSLN WT and KO in both cells grown in culture and in IP tumor tissues using RNA deep sequencing Overall design: Compare and contrast tanscriptome of MSLN WT and KO cells grown in culture and IP tumors tissues using RNAseq The parent KLM1 cell line was crispr edited to make the following cell lines Mock, KO#1 and KO#2. The KO lackMesothelin. These same human-derived cell lines were put into nude mice to form intraperitoneal tumors.