Comparative transcriptome analysis of cadmium responses in Lolium multiflorum. Comparative transcriptome analysis of cadmium responses in Lolium multiflorum

Heavy metal cadmium (Cd) affects seriously crop growth, quality and yield, and has potential threats to human safety. We found the difference of two ryegrasses (Lolium multiflorum Lam.), a high-Cd tolerance (LmHC) and a low-Cd tolerance (LmLC) cultivars , in response to cadmium stress. The germination rate, plant growth, and fresh weight of LmHC were much better than that of LmLC. So we use RNA sequencing to investigate the differentially expressed genes(DEGs) about cadmium response between LmHC and LmLC. Real-time quantitative PCR analysis showed that the same DEGs of LmLC and LmHC had obviously difference expression level under Cd treatment. It was suggestion that differential expression of DEGs might be involved in regulating Cd tolerance, accumulation and translocation.These results will be helpful for understanding the underlying molecular mechanism in Cd toxicity and provide references for improving the ecological environment through genetic improvement. Overall design: RNA sequencing was performed to investigate the differentially expressed genes about cadmium transporter between Lolium multiflorum High Cadmium (LmHC) and Lolium multiflorum Low Cadmium (LmLC). mRNA profiles of two Lolium multiflorum with cadmium treatment 0h and 24h were generated by deep sequencing. The sequence reads that passed quality filters were analyzed at the transcript.