microRNA expression profile of MCF7 cells with retroviral inducible expression of ΔNp63α [miRNA]. Homo sapiens

MCF7 is a breast adenocarcinoma cell line which exhibits properties of differentiated luminal epithelial cells. ΔNp63α, an N-terminally truncated isoform of p51/p63 protein, plays a critical role in mammary gland development. Here, we inducibly express ΔNp63α in MCF7 cells and studied its role in stemness of breast cancer cells. Overall design: Expression of ΔNp63α was induced in MCF7 cells with Doxycycline (Dox) for 48 hours. Total RNA containing microRNAs was isolated. microRNA microarray analysis was performed in Dox- (MCF7-PTO-ΔNp63α-Dox-) and Dox+ (MCF7-PTO-ΔNp63α-Dox+) samples.