The beef cattle rumen microbiome composition is influenced by host genetics

DNA was extracted from the rumen samples using the PowerMag Soil DNA isolation kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer’s protocol with a few modifications as described by publication. The modified protocol also included adding RNase A to the lysis solution to ensure removal of RNA during DNA extraction. The isolated DNA was stored at -20°C until used for bacterial community analysis. The V4 region of the 16S rRNA gene was amplified and sequenced on the MiSeq platform.

采用PowerMag土壤DNA提取试剂盒（MoBio Laboratories，卡尔斯巴德，加利福尼亚州，美国）从瘤胃样本中提取DNA，遵循制造商的方案并进行了一些修改，具体修改内容见相关出版物。修改后的方案还包括向裂解液中添加RNase A，以确保在DNA提取过程中彻底去除RNA。提取的DNA在-20°C条件下储存，直至用于细菌群落分析。在MiSeq平台上对16S rRNA基因的V4区域进行扩增和测序。