Spleen transcriptomes during behavioural fever in Salmo salar

The transcriptomic analysis was evaluated using RNA-seq of salmon challenged with the birnavirus IPNv under two different thermal conditions: (a) constant temperature (mean temperature 15 ± 0.9 ºC, “No-Fever”) and (b) temperature gradient (mean temperature 15 ± 7.4 ºC, “Fever”). In parallel, fish in another tank were treated by adding 100 ml virus free cell culture supernatant to the water (mean temperature 15 ± 7.4 ºC, "mock-infected"). We identified a specific transcriptomic signature for each group in the RNA-seq data and identifying a set of key components that control inflammatory modulation during behavioural fever upon viral infection in mobile ectotherms. Overall design: Spleen mRNA profiles were generated by deep sequencing, in triplicate, using Illumina MiSeq. Tisue were obtained from parr Salmo salar at 24, 48, 72- and 96-hours post infection with IPNv and under two different thermal conditions: (a) constant temperature (mean temperature 15 ± 0.9 ºC, “No-Fever”) and (b) temperature gradient (mean temperature 15 ± 7.4 ºC, “Fever”). In parallel, another spleen samples were obtained from salmon exposed at virus-free cell culture supernatant and temperature gradient (mean temperature 15 ± 7.4 ºC, “Mock”).