Anterograde visualization of projections from orbitofrontal cortex in rat (v1.1)

The project was initiated to determine the projections of the orbital frontal cortex (OFC) to the parahippocampal region in the rat, using the anterograde tracers biotinylated dextran amine (BDA) and Phaseolus vulgaris-leucoagglutinin (PHA-L). The anterograde tracers were injected into four subdivisions of OFC; the medial (MO), ventral (VO), ventrolateral (VLO) and lateral (LO) orbitofrontal areas. All brains were cut in the coronal plane at 50 µm thickness, and every 6th section was sampled for analysis. Complete series throughout the anterior-posterior extent of each brain form the core of the dataset, with a total of 26 animals successfully injected. Out of those 26, 18 were injected with BDA, 4 injected with PHA-L, and 4 injected with both BDA and PHA-L. Locations of all individual injection sites are annotated in the datasets. All sections are in the correct anterior-posterior order. The data show a topographically organized connectivity from OFC to parahippocampal areas, in addition to all other labeled projections throughout the brain.