Supplemental Data for NKT Study

Supplemental Figure 1. Gating strategy for renal flow cytometric analysis. Representative flow cytometry plots are shown to illustrate the gating strategy used to identify renal immune cell populations. Kidney single-cell suspensions were gated sequentially on singlets, live cells, and CD45⁺ leukocytes. Specific immune subsets were defined as follows: CD11b⁺ myeloid cells, CD11b⁺F4/80⁺CD206⁺ M2-like macrophages, CD4⁺CD25⁺Foxp3⁺ regulatory T cells (Tregs), and CD1d tetramer⁺TCRβ⁺ NKT cells. This gating strategy was applied uniformly across all experimental groups.