Table_2_Decoding the formation of diverse petal colors of Lagerstroemia indica by integrating the data from transcriptome and metabolome.DOCX

Lagerstroemia indica has great economic value due to its ecological, medicinal, and ornamental properties. Because its bloom color is one of the most essential characteristics, research into its color development is a hot topic. In this study, five representative colored cultivars were chosen, each representing a different color, such as white, red, pink, violet, and purple. Fully bloomed flowers were used to detect flavonoids in the petals. Anthocyanin is the main factor for the color formation of L. indica. 14 anthocyanins were discovered among the 299 flavonoids. Among 14 anthocyanins, malvidin-3,5-di-O-glucoside varied greatly among four colored samples and is the main contributor to color diversity. Transcriptome sequencing revealed that compared to white flowers, Anthocyanin pathway genes appear to be more active in colored samples. Analyzing the correlation network between metabolites and differential expressed genes, 53 key structural genes, and 24 TFs were detected that may play an essential role in the formation of color in L. indica flowers. Among these, the differential expression of F3′5′H and F3′H between all samples are contributors to color diversity. These findings lay the foundation for discovering the molecular mechanism of L. indica flower color diversity.

由于其在生态、药用和观赏方面的显著经济价值，龙船花（Lagerstroemia indica）备受重视。鉴于其花色为最重要的特征之一，对其色彩形成机制的研究成为热门课题。本研究选取了五种代表性的彩色品种，分别代表白色、红色、粉红色、紫色和紫色等不同颜色。利用完全开放的花朵检测花瓣中的黄酮类化合物。花青素是龙船花（L. indica）色彩形成的主要因素。在299种黄酮类化合物中发现了14种花青素。在这14种花青素中，malvidin-3,5-di-O-glucoside在四种彩色样本中差异显著，是导致色彩多样性的主要贡献者。转录组测序显示，与白色花朵相比，花青素途径基因在彩色样本中活性似乎更高。通过分析代谢物与差异表达基因之间的相关性网络，检测到了53个关键结构基因和24个转录因子，这些基因和因子可能在龙船花（L. indica）花朵色彩形成中发挥至关重要的作用。在这些基因中，F3′5′H和F3′H在所有样本中的差异表达是导致色彩多样性的重要因素。这些发现为揭示龙船花（L. indica）花色多样性的分子机制奠定了基础。