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The process of HDAC11 Assay Development: linearity and Km calculation

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NIAID Data Ecosystem2026-05-02 收录
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With previously optimized conditions, the linearity range for HDAC11 was obtained and the Km for the substrate was calculated. Note: 1. In the assay buffer, BSA conc. is 0.5 mg/ml (instead of 0.5%). 2. In the 7.5 ul developer solution, 40 uM of TSA (Trichostatin A) is also included.

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2024-08-02
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