Expression data from ovalbumin (OVA) induced asthma model

MiRNAs are involved in the pathogenesis of bronchial asthma and are involved in the regulation of airway inflammation, airway remodeling and airway hyperreactivity. In this experiment, we constructed OVA asthma model and identified the differentially expressed miRNAs in asthma and normal models by microarray technology, providing a preliminary basis for future studies on the mechanism of asthma. We used microarrays to detail the global program of gene expression in asthma models and identify miRNAs that are differentially expressed in this process. Ovalbumin (OVA) induced asthma model was established in female BALB/c mice. Briefly, mice were sensitized with 200 μL of sensitization solution (OVA 10 μg + Al(OH)3 1mg) by intraperitoneal injection on days 1, 7, and 14. From day 21, mice were challenged with 3% OVA nebulization for 30 min three times a week until day 56. For control, mice were intraperitoneally injected with 200 μL saline on days 1, 7, and 14, and were challenged with the same amount of normal saline from day 21 to day 56.