File S1 - Fatty Aldehydes in Cyanobacteria Are a Metabolically Flexible Precursor for a Diversity of Biofuel Products

Figure S1: Overexpression of Aar leads to a preferential accumulation of free fatty acids over alkanes. Samples were collected 24, 48 and 72 hours post-induction and analyzed by GC for total FAMES (a), pentadecane (b) and heptadecane (c). Figure S2: Purification of h6-AldE (orf0489). AldE containing an N-terminal 6XHis tag was expressed as a soluble protein in E. coli and purified by metal affinity and size exclusion chromatrography. The UV trace of the size exclusion chromatography step shows that AldE elutes at a size consistent with a homodimer (AldE is blue trace; green trace is of MW standards), as has been seen for other class 3 aldehyde dehydrogenases [27]. The inset is an SDS-PAGE gel, with the arrow indicating h6-AldE. Table S1: Plasmids used in these studies. (PDF)