Thomas Maresca, Edward Salmon (2011) CIL:25304, Drosophila melanogaster. CIL. Dataset

Confocal fluorescence time-lapse imaging of a mitotic Drosophila S2 cell expressing GFP-tubulin and treated with 100 nM taxol for 1 h before imaging to induce assembly of monopolar
spindles.
Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 5 in Maresca and Salmon, 2009 J Cell Biol 184:373-281.

对表达GFP-微管蛋白的果蝇S2细胞进行共聚焦荧光时间间隔成像，该细胞在成像前1小时用100 nM紫杉醇处理，以诱导单极纺锤体的组装。图像以每120秒的间隔使用旋转盘共聚焦显微镜在尼康TE-200支架上，配备100倍1.4数值孔径的Plan APO DIC物镜进行记录，并使用Hamamatsu Orca-ER CCD进行采集。详见Maresca和Salmon在2009年《细胞生物学杂志》（J Cell Biol）第184卷第373-281页发表的补充视频5。