Expression profiling of five different xenobiotics using a C. elegans microarray

Using a C. elegans whole genome DNA microarray in this study, the effects of five different xenobiotics on the gene expression of the nematode were investigated. The exposure time for the following five applied compounds beta-NF (5 mg/l), Fla (0.5 mg/l), atrazine (25 mg/l), clofibrate (10 mg/l) and DES (0.5 mg/l) was 48+/-5 h. The analysis of the data showed a clear induction of 203 genes belonging to different families like the cytochromes P450, UDP-glucoronosyltransferases (UDPGT), glutathione S-transferases (GST), carboxylesterases, collagenes, C-type lectins and others. Under the applied conditions, fluoranthene was able to induce most of the induceable genes, followed by clofibrate, atrazine, beta-naphthoflavone and diethylstilbestrol. A decreased expression could be shown for 153 genes with atrazine having the strongest effect followed by fluoranthene, diethylstilbestrol, beta-naphthoflavone and clofibrate. For upregulated genes a change ranging from approximately 2.1- till 42.3-fold and for downregulated genes from approximately 2.1 till 6.6-fold of gene expression could be affected through the applied xenobiotics. Sample Treatments (by exptids) Clofibrate: 14317, 16443, 16505 Fluoranthene: 33664, 33667, 33669, 23484 beta-Naphthoflavone: 6844, 14320, 14316 Atrazin: 33672, 33674, 23487, 23486 DES: 33671, 23485 A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Keywords: compound_treatment_design本研究采用秀丽隐杆线虫（C. elegans）全基因组DNA微阵列（DNA microarray）技术，探究了五种不同外源性物质（xenobiotics）对该线虫基因表达的影响。五种受试化合物分别为β-萘黄酮（beta-NF，5 mg/l）、荧蒽（Fla，0.5 mg/l）、阿特拉津（atrazine，25 mg/l）、氯贝丁酯（clofibrate，10 mg/l）与己烯雌酚（DES，0.5 mg/l），暴露时长为48±5小时。数据解析结果显示，共计203个基因被显著诱导，这些基因分属多个家族，包括细胞色素P450（cytochromes P450）、UDP-葡萄糖醛酸转移酶（UDP-glucoronosyltransferases, UDPGT）、谷胱甘肽S-转移酶（glutathione S-transferases, GST）、羧酸酯酶、胶原蛋白、C型凝集素（C-type lectins）等。在本实验设置的处理条件下，荧蒽对可诱导基因的诱导效果最为显著，后续依次为氯贝丁酯、阿特拉津、β-萘黄酮与己烯雌酚。同时，共有153个基因的表达量出现下调，其中阿特拉津的下调作用最强，随后依次为荧蒽、己烯雌酚、β-萘黄酮与氯贝丁酯。经上述外源性物质处理后，上调基因的表达变化倍数范围约为2.1至42.3倍，下调基因的表达变化倍数范围约为2.1至6.6倍。样本处理（按实验编号分组）：氯贝丁酯：14317、16443、16505；荧蒽：33664、33667、33669、23484；β-萘黄酮：6844、14320、14316；阿特拉津：33672、33674、23487、23486；己烯雌酚（DES）：33671、23485。该化合物处理设计类型指针对化合物或化学物质（包括激素等生物活性化合物）的给药响应开展检测的实验设计类型。关键词：化合物处理设计（compound_treatment_design）