Increased expression of some toxins in macrophages.
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mRNA was isolated from Salmonella-infected bone marrow derived macrophages pre-treated with IFNγ. The level of toxin mRNA was monitored by semi-quantitative RT-PCR and fold change relative to 16S RNA is shown. mRNA from relE-4 was not detected. Mean of > 3 independent experiments performed in triplicate are shown; error bars denote SEM. Two tailed Student’s t-test comparing macrophage expression to expression in DMEM, P 0.05 (*), P 0.0001 (***). Growth of S. Typhimurium in DMEM and LB were equivalent.
创建时间：
2016-02-22
