Isolation, Structures, and Biological Activities of Triterpenoids from a Penares sp. Marine Sponge

Six new triterpenoids (1–6) and
the previously known penasterone, acetylpenasterol, and ergosta-4,24(28)-dien-3-one
were isolated from a Penares sp. sponge collected
from Vietnamese waters. Structures of the obtained compounds were
established by extensive 1D and 2D NMR spectroscopy and mass spectrometry.
Configurations of the triterpene epoxy lactones (1–4) were determined on the basis of NOESY and CD data and calculation
of spin coupling constants and confirmed by X-ray crystallographic
analysis of compound 2. The isolated triterpenoid 6 was cytotoxic against human leukemia HL-60 cells (IC50 = 9.7 μM).

从越南海域采集到的Penares sp.海绵中分离出六种新的三萜类化合物（1-6）以及已知的penasterone、acetylpenasterol和ergosta-4,24(28)-dien-3-one。通过广泛的核磁共振波谱（1D和2D NMR）和质量谱（mass spectrometry）技术确定了所得化合物的结构。基于核Overhauser效应光谱（NOESY）和圆二色谱（CD）数据、自旋耦合常数计算，确定了三萜环氧内酯（1-4）的构型，并通过化合物2的X射线晶体学分析得到了证实。分离得到的化合物6对人类白血病HL-60细胞具有细胞毒性（IC50 = 9.7 μM）。