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454 pyrosequencing analysis of bacterial communities in human atherosclerotic plaques

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NIAID Data Ecosystem2026-03-10 收录
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Atherosclerotic plaques were removed by endarterectomy from asymptomatic and symptomatic patients. The plaques were divided into three regions known to display different histological vulnerability: A, upstream of maximum stenosis; B, site of maximum stenosis; C: downstream of maximum stenosis. To determine bacterial composition, DNA was isolated from each plaque region and 16S rRNA genes were amplified with PCR, followed by 454 pyrosequencing.

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2018-02-21
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