RNA-seq to profile the alterations in transcript half-lives regarding to ac4C regulation

NAT10-catalyzed N4-acetylcytidine (ac4C) has emerged as a vital post-transcriptional modulator on the coding transcriptome by promoting mRNA stability. To investigate the effect of NAT10-mediated ac4C acetylation modification on RNA stability at the transcriptome scale, we performed high throughput RNA sequencing experiments of control and NAT10 KD hESCs at three time points each with 3 replicates. Overall design: Comparative RNA stability analysis of RNA-seq data for control and NAT10 KD hESCs.