Sicd2 mouse hippocampus exon array analysis

We used Affymetrix Mouse Exon 1.0 ST arrays to identify potential changes in alternative splicing between a subcongenic interval of the QTL Sicd2 on Chr 15 and their FVB-like littermates. Results implicate novel candidate genes conferring susceptibility to seizure-induced cell death. Total RNA was extracted from the hippocampus of 8 Sicd2-ISCL4 subcongenic mice and 8 FVB-like littermates.本研究采用Affymetrix小鼠外显子1.0 ST基因芯片（Mouse Exon 1.0 ST arrays），对15号染色体上数量性状位点（Quantitative Trait Locus, QTL）Sicd2的亚同源导入系区间小鼠，与其同窝FVB品系相似仔鼠之间的可变剪接（alternative splicing）潜在变化进行了鉴定。研究结果揭示了若干可赋予惊厥诱导细胞死亡（seizure-induced cell death）易感性的新型候选基因。本研究从8只Sicd2-ISCL4亚同源导入系小鼠及8只同窝FVB品系相似仔鼠的海马体（hippocampus）中提取了总RNA（Total RNA）。