File S1 - Structure-Function Relationship of Substituted Bromomethylcoumarins in Nucleoside Specificity of RNA Alkylation
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Contains: Table S1: Retention times, mass transitions and correction factors of BMB nucleoside conjugates. Table S2: Retention times, mass transitions and correction factors of compound 2 nucleoside conjugates. Table S3: Retention times, mass transitions and correction factors of compound 3 nucleoside conjugates. Table S4: Retention times, mass transitions and correction factors of compound 4 nucleoside conjugates. Table S5: Retention times, mass transitions and correction factors of compound 5 nucleoside conjugates. Table S6: Retention times, mass transitions and correction factors of compound 6 nucleoside conjugates. Table S7: Overview of all correction factors and standard deviations. Table S8: Analysis of tRNA composition. Figure S1: Gel analysis of tRNA in-vitro-transcript (IVT) and tRNA E. coli. Figure S2: Major base composition is not altered upon coumarin treatment. Figure S3: UV spectrometrical changes in absorption by pseudouridine alkylation at different pH. (DOCX)



