Relationship between S100A8 or S100A9 expression and clinicopathological parameters of patients.

CRC, colorectal carcinoma samples; normal tissues, matching distal normal tissues; n, number of samples. The proportion of staining of tumor cells was classified in four grades (<10% = 1, 10–25% = 2, 26–75% = 3, >75% = 4). In addition, its staining intensity was also divided into four subgroups (no staining = 0, weak = 1, moderate = 2, strong = 3). Staining index (value range, 0–12) = proportion×intensity. The expression of S100A8 and S100A9 was finally analyzed by a simplified score (scoreI, staining index 0–1; scoreII, staining index 2–4; score III, staining index 6–8; score IV, staining index 9–12). The expression of S100A8 and S100A9 in tumors with staining score of at least II were regards as positive. a, Pearson chi-squared test; b, Yates-corrected chi-squared test. *p-value<0.05 was considered significant.