Effect of depletion of ALKBH5 on transcriptional landscape in bone marrow neutrophils from septic mice

By performing RNA-seq analysis on bone marrow neutrophils from the Alkbh5-deficient mice and Wild-type littermates undergoing CLP-induced sepsis, we want to investigate the effect of ALKBH5 on transcriptional landscape of mouse bone marrow neutrophils during bacterial infection. Then, we performed gene expression profiling and Gene Ontology enrichment analysis of the significantly differentially expressed genes using data obtained from RNA-seq. Comparative gene expression profiling analysis of RNA-seq data for bone marrow neutrophils from Alkbh5-deficient mice (Alkbh5-/-, KO) and Wild-type littermates (WT) at 12h after CLP.