Effects Of Simulated Space Conditions On CD4+ T Cells: A Multi Modal Analysis

The goal of this study was to isolate CD4+ T cells from the blood of healthy volunteers and expose them to simulated space stressors in a single and combined manner to investigate their effects on T cell activation, gene expression, and cytokine production. Cytokine levels for Th1, Th2, and Treg cell populations were measured using multiplex Luminex assays to assess functional changes in T cell subsets under simulated space conditions. Furthermore, this study aimed to analyze gene expression patterns using RNA-seq to identify key genes and pathways involved in T cell activation and the immune response that are significantly altered in response to simulated space conditions. Proteomic analysis was also conducted to examine changes in protein expression and modifications that may occur in CD4+ T cells under simulated space conditions. Overall, this study aimed to provide a comprehensive understanding of the effects of simulated space conditions on CD4+ T cells and their role in the immune response.