Transcriptome analysis of human erythroid cells differentiated from iPSCs, cord blood and adult peripheral blood

The aim of the study is to develop a scalable continuous agitation suspension culture bioprocess for differentiation of hiPSCs towards erythroid cells. Using process optimization, we developed the entire process completely in agitation suspension culture condition starting from hiPSC expansion through to hematopoietic induction and erythroid differentiation. Transcriptome profiling was carried out to facilitate comparisons between erythroid cells differentiated from hiPSCs using this bioprocess, and erythroid cells differentiated from cord blood and adult CD34+ cells. A total of 11 samples were analyzed, consisting of 5 replicates of erythroid cells differentiated from hiPSCs in stirred spinner flasks, 3 replicates of cord blood differentiated erythroid cells, and 3 replicates of adult peripheral blood differentiated erythroid cells.