gmMφ express high FcγRIIb levels, while mMφ express more FcγRIIa and FcγRIII.
收藏Figshare2016-02-23 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_gmM_966_express_high_Fc_947_RIIb_levels_while_mM_966_express_more_Fc_947_RIIa_and_Fc_947_RIII_/1219576
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Monocytes were cultured for 6 days with GM-CSF or M-CSF into gmMφ and mMφ respectively. Expression of CD14, CD163 and MHC-II (A) and all FcγRs (B) was determined by flow cytometry. (B) For FcγR expression, representative FACS plots are shown together with bar graphs showing mean (and SEM of) MFI and percentage of positive cells from 11 donors. Histograms show isotype control (thin line) and FcγR specific antibody (solid grey). (C) mRNA expression of FcγRIIb1 and FcγRIIb2 were determined by qPCR and plotted as relative expression compared to GAPDH. Bars are mean and SEM of 7 donors. (D) gmMφ and mMφ were incubated with FITC-labeled ICs (50 µg/ml) for 30 min at 4°C for binding and 37°C for uptake. IC uptake was determined in the presence of trypan blue. Bars are mean and SEM from 4 donors. *P
创建时间：
2016-02-23
