Thermodynamic values from van’t Hoff analysis.

Data are in kJ/mol unless otherwise specified. *Bold-face regions indicate substitutions at the functional epitope for BO2C11, i.e. where the substitution caused a greater than fourfold increase in kd relative to WT-FVIII-C2 binding. †The standard errors (SE) of ΔHAº and TΔSAºare based on the SE of the slope and intercept, respectively. Reported errors for ΔΔHAº, Δ(TΔSAº) and ΔΔGAº§ are the square roots of the sums of the squares of the errors. ‡The SE of ΔGAº was derived from the SE of fitted values from the linear regression (van’t Hoff analysis), transformed to the ΔGAº scale (e.g. SE (ln(KD)/T = 298)xRT/1000) §The ΔGAº and ΔΔGAº errors were all less than one kJ/mol but are reported here as “±1” for consistency with the significant figures of the measured data. ||The KD values from the earlier kinetic runs at 25oC only (final column) are included for comparison with KD values derived from the ΔGAºof SPR runs carried out at several temperatures. Thermodynamic values from van’t Hoff analysis.