STIL overexpression shortens lifespan and reduces tumor formation in mice

Raw sequencing data from transgenic mouse models with constitutive, global STIL overexpression as well as from transgenic mouse models with inducible, skin-specific STIL overexpression. Whole genome sequencing (WGS) was performed to analyze the levels of chromosomal copy number alterations whereas RNA sequencing data were generated to analyze a chromosomal instability (CIN) score. Overexpression of the structural centrosome protein STIL induced centrosome amplification accompanied by aneuploidy, which strongly impaired cell proliferation and viability. Constitutive STIL overexpression from embryonal development onwards caused microcephaly, perinatal lethality and reduced life span in mice. Importantly, both overall tumor formation in mice with constitutive, global STIL overexpression and chemical skin carcinogenesis in animals with inducible, skin-specific STIL overexpression were reduced, an effect that was not rescued by concomitant interference with p53 function.