Kinetic constants using the substrate N-benzoyl-Phe-Val-Arg-pNA. Specific activity using azocasein and IC50 value for the inhibitor E-64.
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aStandard errors were calculated based on nonlinear fitting of the Michaelis–Menten saturation curve using the software Graphpad PRISM (http://www.graphpad.com/prism).bEach value of specific activity of each enzyme is a mean of three independent experiments ± SD.
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2015-12-02
